science
Grade-10
Easy

Question

DNA is sensitive to temperatures above _______.

  1. 90o C.

  2. 85o C.

  3. 95o C.

  4. 80o C.

hintHint:

  • The temperature at which denaturation occurs in PCR.

The correct answer is:

95o C.


    • PCR or polymerase chain reaction is a in vitro technique for producing multiple copies of DNA
    • DNA is double stranded helix which contains sequence of nucleotide complementary to each other
    • DNA is subjected to multiple cycles of three stages in PCR which include:
    1. Denaturation
    2. Annealing
    3. Extension
    • During denaturation, the double strand of DNA is separated into two single strands by subjecting the DNA to a temperature of 95oC
    • During denaturation, the separation of the strands occur due to breaking of hydrogen bonds between the two complementary stands 

    • PCR was discovered by Kary Mullis
    • Various forms of PCR are available which are all based on the basic three steps 
    • Besides replication, PCR can also be used in diagnosis, modification of genetic material, paternity testing and many more

    Related Questions to study

    Grade-10
    science

    Ionizing radiations include:

    • Mutagens are the factors that cause mutation
    • Physical mutagens include ionizing radiations, high concentration of carbon dioxide, etc

    Ionizing radiations include:

    scienceGrade-10

    • Mutagens are the factors that cause mutation
    • Physical mutagens include ionizing radiations, high concentration of carbon dioxide, etc

    Grade-10
    science

    Generally, the mutations are:

    • In harmful mutation, due to insertion or deletion of a base the resulting protein synthesis become faulty
    • In silent mutation, the alteration in base sequence is such that the final protein is not changed

    Generally, the mutations are:

    scienceGrade-10

    • In harmful mutation, due to insertion or deletion of a base the resulting protein synthesis become faulty
    • In silent mutation, the alteration in base sequence is such that the final protein is not changed

    Grade-10
    science

    Which of the following base analogs exhibit tautomerism?

    • Base analogues have structure similar to the common bases (Adenine, guanine, cytosine and thymine)
    • 5bromouracil is the base analogue for thymine

    Which of the following base analogs exhibit tautomerism?

    scienceGrade-10

    • Base analogues have structure similar to the common bases (Adenine, guanine, cytosine and thymine)
    • 5bromouracil is the base analogue for thymine

    parallel
    Grade-10
    science

    Point Mutation is caused by:

    • Point mutation can be caused due to substitution by a base analogue or due to exposure to ionizing radiations as well
    • Point mutation can also be due to deletion or insertion of a base pair

    Point Mutation is caused by:

    scienceGrade-10

    • Point mutation can be caused due to substitution by a base analogue or due to exposure to ionizing radiations as well
    • Point mutation can also be due to deletion or insertion of a base pair

    Grade-10
    science

    Examples of base analogs are ________.

    • Base analogues are naturally occurring molecules having similar structure to common bases
    • 5BU has structure similar to thymine
    • 2aminopurine has structure similar to purine

    Examples of base analogs are ________.

    scienceGrade-10

    • Base analogues are naturally occurring molecules having similar structure to common bases
    • 5BU has structure similar to thymine
    • 2aminopurine has structure similar to purine

    Grade-10
    science

    Alkyl bases are induced in DNA by which chemical mutagen?

    • The alkylating agents helps in crosslinking of DNA which cannot replicate 
    • Other mechanism is by replacing hydrogen atoms of DNA with alkyl groups which cannot be repaired by the cell

    Alkyl bases are induced in DNA by which chemical mutagen?

    scienceGrade-10

    • The alkylating agents helps in crosslinking of DNA which cannot replicate 
    • Other mechanism is by replacing hydrogen atoms of DNA with alkyl groups which cannot be repaired by the cell

    parallel
    Grade-10
    science

    Base analogs are which type of mutagens?

    • Bases are classified into purine and pyrimidine
    • Purine include adenine and guanine which are two ringed structures
    • Pyrimidine include thymine and uracil which are single ringed structures

    Base analogs are which type of mutagens?

    scienceGrade-10

    • Bases are classified into purine and pyrimidine
    • Purine include adenine and guanine which are two ringed structures
    • Pyrimidine include thymine and uracil which are single ringed structures

    Grade-10
    science

    Ionizing radiation causes ___________.

    • Ionizing radiations have shorter wavelength but high energy
    • Ionizing radiations cause single strand break or crosslinking

    Ionizing radiation causes ___________.

    scienceGrade-10

    • Ionizing radiations have shorter wavelength but high energy
    • Ionizing radiations cause single strand break or crosslinking

    Grade-10
    science

    If the mutation occurs in the non-functional part of DNA, then these mutations may remain as _________.

    • Silent mutation does not affect the individual since the change in the nucleic acid sequence doe not alter the amino acid produced.

    If the mutation occurs in the non-functional part of DNA, then these mutations may remain as _________.

    scienceGrade-10

    • Silent mutation does not affect the individual since the change in the nucleic acid sequence doe not alter the amino acid produced.

    parallel
    Grade-10
    science

    Mutations are mainly responsible for:

    • Not all mutations are harmful
    • Some mutations are silent, having no effect
    • Development of lactose tolerance is an example for beneficial mutation

    Mutations are mainly responsible for:

    scienceGrade-10

    • Not all mutations are harmful
    • Some mutations are silent, having no effect
    • Development of lactose tolerance is an example for beneficial mutation

    Grade-10
    science

    _______ is the most mutagenic among UV rays.

    • UV B and UV C have mutagenic effects
    • The UV C radiations are filtered by the atmosphere
    • Transition mutation is caused by UV C

    _______ is the most mutagenic among UV rays.

    scienceGrade-10

    • UV B and UV C have mutagenic effects
    • The UV C radiations are filtered by the atmosphere
    • Transition mutation is caused by UV C

    Grade-10
    science

    __________is the degree by which differences are seen in parents and their off-springs.

    • No two individual is alike due to variation
    • Variation is caused due to combination of paternal and maternal genes
    • Environmental factors also can influence variation in some cases

    __________is the degree by which differences are seen in parents and their off-springs.

    scienceGrade-10

    • No two individual is alike due to variation
    • Variation is caused due to combination of paternal and maternal genes
    • Environmental factors also can influence variation in some cases

    parallel
    Grade-10
    science

    Physical mutagens include:

    • Mutation cause change in DNA sequence
    • Not all mutations are harmful
    • Some mutations are beneficial to survive, while some mutations may not produce any effect

    Physical mutagens include:

    scienceGrade-10

    • Mutation cause change in DNA sequence
    • Not all mutations are harmful
    • Some mutations are beneficial to survive, while some mutations may not produce any effect

    Grade-10
    science

    When a gene pair hides the effect of another gene, the phenomenon is called ____________.

    • Genes are specific DNA sequence coding for a particular trait
    • The phenotype of an individual is determined by the genotype
    • The genotype is represented by the two alleles

    When a gene pair hides the effect of another gene, the phenomenon is called ____________.

    scienceGrade-10

    • Genes are specific DNA sequence coding for a particular trait
    • The phenotype of an individual is determined by the genotype
    • The genotype is represented by the two alleles

    Grade-10
    science

    Which of the following traits were not used by Mendel?

    • Mendel used true breeding plants
    • The parent generation were homozygous with contrasting characters
    • The contrasting characters of the parents were dominant and recessive

    Which of the following traits were not used by Mendel?

    scienceGrade-10

    • Mendel used true breeding plants
    • The parent generation were homozygous with contrasting characters
    • The contrasting characters of the parents were dominant and recessive

    parallel

    card img

    With Turito Academy.

    card img

    With Turito Foundation.

    card img

    Get an Expert Advice From Turito.

    Turito Academy

    card img

    With Turito Academy.

    Test Prep

    card img

    With Turito Foundation.