science
Grade-10
Easy
Question
DNA is sensitive to temperatures above _______.
90o C.
85o C.
95o C.
80o C.
90o C.
85o C.
95o C.
80o C.
Hint:
- The temperature at which denaturation occurs in PCR.
The correct answer is: 95o C.
- PCR or polymerase chain reaction is a in vitro technique for producing multiple copies of DNA
- DNA is double stranded helix which contains sequence of nucleotide complementary to each other
- DNA is subjected to multiple cycles of three stages in PCR which include:
- Denaturation
- Annealing
- Extension
- During denaturation, the double strand of DNA is separated into two single strands by subjecting the DNA to a temperature of 95oC
- During denaturation, the separation of the strands occur due to breaking of hydrogen bonds between the two complementary stands
- PCR was discovered by Kary Mullis
- Various forms of PCR are available which are all based on the basic three steps
- Besides replication, PCR can also be used in diagnosis, modification of genetic material, paternity testing and many more
Related Questions to study
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Ionizing radiations include:
- Mutagens are the factors that cause mutation
- Physical mutagens include ionizing radiations, high concentration of carbon dioxide, etc
Ionizing radiations include:
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- Mutagens are the factors that cause mutation
- Physical mutagens include ionizing radiations, high concentration of carbon dioxide, etc
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Generally, the mutations are:
- In harmful mutation, due to insertion or deletion of a base the resulting protein synthesis become faulty
- In silent mutation, the alteration in base sequence is such that the final protein is not changed
Generally, the mutations are:
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- In harmful mutation, due to insertion or deletion of a base the resulting protein synthesis become faulty
- In silent mutation, the alteration in base sequence is such that the final protein is not changed
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Which of the following base analogs exhibit tautomerism?
- Base analogues have structure similar to the common bases (Adenine, guanine, cytosine and thymine)
- 5bromouracil is the base analogue for thymine
Which of the following base analogs exhibit tautomerism?
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- Base analogues have structure similar to the common bases (Adenine, guanine, cytosine and thymine)
- 5bromouracil is the base analogue for thymine
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Point Mutation is caused by:
- Point mutation can be caused due to substitution by a base analogue or due to exposure to ionizing radiations as well
- Point mutation can also be due to deletion or insertion of a base pair
Point Mutation is caused by:
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- Point mutation can be caused due to substitution by a base analogue or due to exposure to ionizing radiations as well
- Point mutation can also be due to deletion or insertion of a base pair
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Examples of base analogs are ________.
- Base analogues are naturally occurring molecules having similar structure to common bases
- 5BU has structure similar to thymine
- 2aminopurine has structure similar to purine
Examples of base analogs are ________.
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- Base analogues are naturally occurring molecules having similar structure to common bases
- 5BU has structure similar to thymine
- 2aminopurine has structure similar to purine
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Alkyl bases are induced in DNA by which chemical mutagen?
- The alkylating agents helps in crosslinking of DNA which cannot replicate
- Other mechanism is by replacing hydrogen atoms of DNA with alkyl groups which cannot be repaired by the cell
Alkyl bases are induced in DNA by which chemical mutagen?
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- The alkylating agents helps in crosslinking of DNA which cannot replicate
- Other mechanism is by replacing hydrogen atoms of DNA with alkyl groups which cannot be repaired by the cell
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Base analogs are which type of mutagens?
- Bases are classified into purine and pyrimidine
- Purine include adenine and guanine which are two ringed structures
- Pyrimidine include thymine and uracil which are single ringed structures
Base analogs are which type of mutagens?
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- Bases are classified into purine and pyrimidine
- Purine include adenine and guanine which are two ringed structures
- Pyrimidine include thymine and uracil which are single ringed structures
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Ionizing radiation causes ___________.
- Ionizing radiations have shorter wavelength but high energy
- Ionizing radiations cause single strand break or crosslinking
Ionizing radiation causes ___________.
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- Ionizing radiations have shorter wavelength but high energy
- Ionizing radiations cause single strand break or crosslinking
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If the mutation occurs in the non-functional part of DNA, then these mutations may remain as _________.
- Silent mutation does not affect the individual since the change in the nucleic acid sequence doe not alter the amino acid produced.
If the mutation occurs in the non-functional part of DNA, then these mutations may remain as _________.
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- Silent mutation does not affect the individual since the change in the nucleic acid sequence doe not alter the amino acid produced.
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Mutations are mainly responsible for:
- Not all mutations are harmful
- Some mutations are silent, having no effect
- Development of lactose tolerance is an example for beneficial mutation
Mutations are mainly responsible for:
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- Not all mutations are harmful
- Some mutations are silent, having no effect
- Development of lactose tolerance is an example for beneficial mutation
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_______ is the most mutagenic among UV rays.
- UV B and UV C have mutagenic effects
- The UV C radiations are filtered by the atmosphere
- Transition mutation is caused by UV C
_______ is the most mutagenic among UV rays.
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- UV B and UV C have mutagenic effects
- The UV C radiations are filtered by the atmosphere
- Transition mutation is caused by UV C
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__________is the degree by which differences are seen in parents and their off-springs.
- No two individual is alike due to variation
- Variation is caused due to combination of paternal and maternal genes
- Environmental factors also can influence variation in some cases
__________is the degree by which differences are seen in parents and their off-springs.
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- No two individual is alike due to variation
- Variation is caused due to combination of paternal and maternal genes
- Environmental factors also can influence variation in some cases
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Physical mutagens include:
- Mutation cause change in DNA sequence
- Not all mutations are harmful
- Some mutations are beneficial to survive, while some mutations may not produce any effect
Physical mutagens include:
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- Mutation cause change in DNA sequence
- Not all mutations are harmful
- Some mutations are beneficial to survive, while some mutations may not produce any effect
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Mutation occurs at the time of:
- Mutation can be due to genetic or environmental factors
- The cause of mutation is called mutagen
- The individual affected is called mutant
- Not all mutations are harmful
Mutation occurs at the time of:
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- Mutation can be due to genetic or environmental factors
- The cause of mutation is called mutagen
- The individual affected is called mutant
- Not all mutations are harmful
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When a gene pair hides the effect of another gene, the phenomenon is called ____________.
- Genes are specific DNA sequence coding for a particular trait
- The phenotype of an individual is determined by the genotype
- The genotype is represented by the two alleles
When a gene pair hides the effect of another gene, the phenomenon is called ____________.
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- Genes are specific DNA sequence coding for a particular trait
- The phenotype of an individual is determined by the genotype
- The genotype is represented by the two alleles
